This study aimed to investigate the effects of enzymatic antioxidants superoxide dismutase (SOD) and catalase supplementation on semen quality of multi-spurs chicken during liquid storage up to 48 h at 4°C. Pooled semen of twelve Vietnamese multi-spurred roosters was randomly divided into 7 experimental groups: control without supplement; SOD supplemented at 100 UI/mLor 200 UI/mL; catalase supplemented at 200 UI/mLor 400 UI/mL, and combination of 100 SOD + 200 catalaseUI/mL or 200 SOD +400 catalaseUI/mL.The highest quality treated semen was selected base on the semen motility, viability, abnormality at 0 h, 24 h, and 48 h of storage, and evaluated the fertility in comparison with control group. The results showed that the motility and viability of stored sperm were increased by the addition of 200 SOD + 400 catalaseUI/mL. Moreover, the fertility of stored chicken sperm was increased (p<0.05) compared to that of control treatment. In conclusion, the supplementation of 200 SOD +400 catalaseUI/mL improved the motility, viability, and fertility of multi-spurs chicken sperm during liquid preservation after 24 h and 48 h.