Research aims to evaluate the activation process of 3 lactic acid bacteria strains on an industrial scale to successfully produce probiotics containing lactic acid bacteria with high applicability in livestock to minimizeantimicrobial resistant of infection bacteria.The activation process was evaluated by diffusion test, lactic acid production and bacteriocin activity.Through the study, we evaluated the activation process of 3 strains of Lactic bacteria. Specifically in all 3 strains of Lactobacillus plantarum UL485; Bifidobacterium animalis NG52 and Pediococcus pentosaceus PT 16.9 were investigated, allstrains have good inhibitory ability and lactic acid content is >13g/liter and bacteriocin activity >1.000AU/ml, proving that the strainshave been well activated and can be used for further procedures.