The aim of this study is to evaluate theeffectiveness of the use of biological mattresses in raising beef cattle for growth, development, gas concentration in the barn, and economic efficiency. The experiment was conducted on 26 pure Senepol cattle imported from Australia divided into 2 batches (Experiment): the experiment and control for each batch were 21 cows aged 14 – 18 months old and had an average weight of 385.31 ± 3.05 kg. The cows in the control batch were raised on a cement base and cleaned daily, the cows in the experimental batch were raised on a biological pad imported from T&T 159 Joint Stock Company with a shelf life of 60 days. During the experiment, the herd was determined the indicators of weight gain at the beginning and end of the experiment and measured NH3, H2S daily in the laboratory every 24 hours with the GX-6000 device at the location between the barn and the economic efficiency determined by the difference between the expenditure and the profit earned. Experimental results show that the use of biological padding in beef cattle breeding has reduced the concentration of H2S and NH3 gases in the barn to improve the environment in the barn to help the herd grow evenly, healthy, and maintain growth. In addition, it helps to save care work, clean cages and bring high economic efficiency