The objective of this study was to evaluate the yield and quality of bird's nests in swiftlet houses in Binh Duong and Binh Phuoc provinces. Eighty swiftlet houses for a province were selected from the 200 surveyed swiftlet houses for a province to evaluate the yield, the percentage of bird's nests, and the nutritional composition of bird's nests. The swiftlet house has an average area of 270.97-275.73 m2 (2-3 floors), and the age of harvest is about 5.24-5.35 years. Temperature, humidity, sound intensity, and light intensity in the swiftlet houses, respectively 27.78 - 27.93°C; 80.75-80.85%; 71.04-71.06 dB and 0.18-0.20 Lux and a number of other factors in the bird's nest are similar. The results show that the yield of bird's nest for a harvest in Binh Duong is 14.19 g/m2; in Binh Phuoc is 12.01 g/m2.The percentage of type I and type II bird's nests in Binh Duong is 30.62% and 46.36% respectively; in Binh Phuoc is 30.41% and 49.16%. The percentage of dry matter, protein, carbohydrates, and total fat of bird's nests in Binh Duong is 84.65%, 57.56%; 22.81%; 0.56% respectively; in Binh Phuoc is 84.53%; 57.68%; 22.59%; 0.53%. In the crude protein composition of bird's nest in two provinces, there are 18 kinds of amino acids. The yield and quality of bird's nests in Binh Duong and Binh Phuoc are equivalent.