This study aimed to assess the current status of antibiotic usage in chicken production on 135 backyard chicken farms in Tra Vinh province. The results showed that the use of antibiotics is relatively common in raising backyard chickens; there are 16 commercial antibiotics, of which Amox-Colis is commonly used (62.22%). This survey revealed that antibiotics were used up to 86.67% for disease prevention and 100% for disease treatment. There are some households using antibiotics to enhance lean meat in chicken production (15.56%). Only 69.63% of households used the dose recommended by the manufacturer; the remaining households used the dose incorrectly. Some households arbitrarily used antibiotics (32.59%) without following a withdrawal period stated on the label to sell chickens (91.11%), and up to 91.85% of households did not send samples for antibiotic susceptibility testing. The uncontrolled use of antibiotics in household chicken production can increase the risk of antibiotic resistance in bacteria, causing economic losses to farmers and affecting public health.