In recent years, the number of ChiemHoa buffaloes has decreased gradually and tends to be inbreeding and seriously degraded. The evaluation and effective using of ChiemHoa buffaloes genetic resourses help improve the quality of ChiemHoa buffalo population. The study evaluated the genetic characteristics of 10 ChiemHoa male buffaloes for breeding in Tuyen Quang and Mountainous Animal Husbandry Research and Development Center (Song Cong, Thai Nguyen province) from 2019-2021 by 8 microsatellite markers, CSSM057, CSSM029, ILST033, RM099, CSSM022, BMC1013, ETH003, CSSM038. The purpose of the study is to evaluate the genetic distance of the Chiem Hoa buffalo population, thereby orienting the use and exploitation of effective buffalo genetic resources in the future. The results of the buffalo alleles showed that there were 34 polymorphic bands and 4 single-bands. Among these results, 6 microsatellite markers for these buffaloes showed high genetic diversity; ILST033 and BMC1013 markers had average genetic diversity. The genetic distance of these buffaloes range from 0.364 to 0,909 and the average was 0.6. The 205 and 206 buffalo had highest genetic distance; the 03 and 05 buffaloes had lowest genetic distance. These results suggested these buffaloes were high diversity and could use for breeding in Tuyen Quang province.