The experiment was conducted to estimate the optimal level of metabolizable energy (ME) and standardized ileal digestible lysine (SID Lys) for sows during gestation. A total of 90 PS gestating sows (Landrace × Yorkshire and Yorkshire × Landrace) were used in two-factorial experimental design. The first factor is ME (three levels: 2,900; 3,000 and 3,100 kcal/kg) and the second is SID Lys (three levels: 0.50; 0.60; 0.70%) for 9 treatments in 10 replications with one sow per replicate for each treatment. The experiment was carried out at Thong Nhat farm, Cu Chi district, Ho Chi Minh City from March to August in 2019. The results showed that ME at 3,000 kcal/kg, 0.60 and 0.70% SID Lys in diet were likely to be a right level for PS gestating sows in early and late pregnancy, respectively.