The research was conducted to produce and preserve semen from 05 buffalo bulls, selected from the Chiem Hoa buffalo herd in 2018. The research site is at the Mountainous Livestock Research and Development Center. The female buffalo was bred with artificial insemination in Chiem Hoa district - Tuyen Quang and Thai Nguyen. Research period is from January 2019 to December 2020. Research content: Evaluating the production ability and quality of straw of semen frozen produced from Chiem Hoa buffalo semen; Insemination and fertility assessment of straw semen when mating on female buffalo herds in Tuyen Quang and Thai Nguyen. The study results showed that: The average number of standard straws produced is 127.94 straws per one time semen production (ranging from 88.41 to 199.70 straws per one time semen production). Chiem Hoa buffalo straw semen has good quality. The sperm motility after freezing is 48.85% in average; sperm post thaw motility after 12 months of preservation decreased 0.16%. The conception rate at the first artificial insemination of the Chiem Hoa buffalo semen frozen with the local female buffalo reached 50.67%. It is rated well, meeting current local breeding requirements.