The study aimed to evalute effects of treating Mycoplasma suis in pigs by different kinds of antibiotics on a total of 100 post-weaned pigs, which had confirmed positive with Mycoplasma suis, were divided into 4 lots. These pigs of four lots of trial was treated by antibiotics addition to the feed: lot I (flofernicol and doxycycline), lot II (doxycycline), lot III (bactrim), lot IV (doxycycline and bactrim), the drug was given twice, for one week each time, separately one month during the period from post-weaning to 3-month age. The results, were tested by observing Giemsa-stained blood smears and polymerase chain reaction (PCR) testing, showed antibiotic apply had a good effect on reducing the M. suis infection intensity, decreased from 2.4 - 3.2 to 1.4 - 2.2 points by observing Giemsa-stained blood smears, and positive ratio of PCR testing decreased from 95% to 45%. At the same time, the results also showed that the use of doxycycline and other broad-spectrum antibiotic combination (Lot IV and I) was more effective than treatment without combination (lot II and lot III) and lot IV showed the best improvement in the treatmentof M. suis infection.