Genetic polymorphisms of some candidate genes associated with reproduction and growth traits in Hung and Meo indigenous pigs
The purpose of this study was to evaluate allele and genotype frequencies of OVGP1, LIF polymorphic sites related to fertility traits, and GH and IGF1 polymorphisms associated with growth traits in Hung and Meo pig breeds. The study was conducted on 84 Hung sows and 86 Meo sows; 86 Hung and 85 Meo finishing pigs by PCR-RFLP method. It was shown that the OVGP1 gene has 3 genotypes, namely AA, AB, and BB, with the frequency of allele A and B in Meo pigs being 0.783 and 0.217 and in Hung pigs being 0.720 and 0.280. The LIF gene also had 3 genotypes: CC, CT, and TT; the frequency of T and C alleles in Meo pigs was 0.913 and 0.087, respectively and in Hung pigs the corresponding values were 0.880 and 0.120. In addition, 3 genotypes, namely AA, AB, BB were detected in the GH gene, of which the frequency of A and B alleles were 0.700 and 0.300 in Meo pigs and 0.531 and 0.469 in Hung pigs. Finally, AA and AB genotypes were found in the IGF1 gene, with the frequency of allele A and B in Meo pig being 0.553 and 0.447 and in Hung pig being 0.826 and 0.174. Besides, it was shown that at the OVGP1 and LIF loci, the frequencies in Meo breed were in Hardy-Weinberg equilibrium (OVGP1, P=0.339 and P=0.068, respectively). Similar results were also found in the Hung pig breed (OVGP1, P=0.053 and P=0.314, respectively). Moreover, in the Meo pig breed, the genotype frequencies in GH gene were balanced (P=0.680>0.05), whereas those of the IGF1 gene were not (P=0.000<0.05). For the remained polymorphic sites in GH and IGF1 of Hung pig breed, the genotype frequencies were in Hardy-Weinberg equilibrium (P>0.05).
