Polymorphisms of candidate genes and their association with intramuscular fat in Duroc pig
This study aimed to determine single nucleotide polymorphisms (SNPs) in four candidate genes: Fatty Acid Binding Protein 3 (FABP3), Adrenoceptor Beta 3 (ADRB3), Perilipin 2 (PLIN2), and Acyl-CoA Synthetase Long-Chain Family Member 4 (ACSL4) as well as anassociation between these SNPs and intramuscular fat (IMF) in Duroc pig population. A total of 200 pigs was collected, and DNA samples were extracted. The PCR-RFLP method was used to analyze five SNPs, including FABP3-HinfI, FABP3-BsrfI, ADRB3-TaqI, PLIN2-Mva1269I and ACSL4-RsaI.A General Linear Model was used to evaluate the association between SNPs and IMF. Results estimated the frequency of alleles and genotypes for SNPs in these candidate genes. While only two genotypes were found in three SNPs, FABP3/HinfI, PLIN2/Mva1269I, and ACSL4/RsaI, SNP at ADRB3/TaqI indicated three different genotypes, and the SNP at FABP3/BsrfI was homozygous. All homozygous genotypes of SNPs accounted for higher frequency except the SNP of ADRB3/TaqI. However, they had no significant association between the SNPs and the IMF in experimenting with the Duroc pig population.
