Research to determine dilution medium and methods of freezing sheep semen in the production rams frozen semen
The experiment was conducted on 600 semen samples of 10 rams (05 Phan Rang and 05 Dorper rams) which are 14-17 months, to determine the extenders and the freezing semen methods to produce ram frozen semen. There are three different extenders (CUE; TRIS and VNE), and three different freezing methods (slow freezing, programmed temperature - P1; quick freezing, programmable temperature - P2 and quick freezing, manually - P3). The results showed that the VNE extender gives the best quality of sperm after thawing (P <0.05). Then the results of TRIS and CUE, respectively. Namely motility was 47.76%; 43.69% and 37.53%; abnormal sperm was 22.40%; 23.13% and 24.12%; The rate of live sperm reached 68.54%; 65.63% and 63.05%. The quick freezing method, programmed temperature (P2) has the best sperm quality after thawing such as motility, the rate of abnormal sperm and live sperm (P <0.05). Method P2 showed the post-thaw semen qulity result that motility reached 48.66%; the rate of abnormal sperm was 24.18%; rate of live sperm was 67.52%.
