This study aimed to investigate the effects of probiotics supplementation in bedding on growth and reproductive performance of Hoa Lan ducks. The study was carried out from 10/2018 to 2/2020 at a duck household in Tien Giang province. 1 day-old ducklings were aranged in 2 groups, in each group there were 50 males and 200 females, 3 repetitions. Group 1 (control group): None supplementation and Group 2 (experimental group): Supplemention of probiotics in bedding. The results showed that during 72 weeks of raising, density of E.coli and Salmonella were greatly reduced, survival rate of male and female ducks were increased 2.58% và 1.45%, rate of egg-laying was higher 0.87% in the experimental group comparing to the control group. Egg production/hen/52 weeks of laying reached 218.79 egg and higher 3.16 egg than in the control group. The everage of egg weight in experimental group was 72.65g/egg, while 71.88 g/egg in control group. FCR/10egg in experimental group reduced 0.11kg. The rate of embryo and hatching in experimental group showed higher than in control group. The experimental group increased economic efficiency to farmer (8.1%) as compare with control group.