The study aimed to evaluate the effects of Actisaf product (Saccharomyces cerevisiae sc 47) supplemented on egg yield and egg quanlity of layer Star 53 ducks. The study was carried out from August, 2019 to February, 2020 at the duck farm of Hai Thinh feed joint stock company, Quang Yen town branch, Quang Ninh province. In this experiment, 600 ducks were devided into two groups (experimental group supplemented bioproduct Probiotic Actisaf with the rate of 0.5g/kg feed and control group not used), with 3 replicates and 100 ducks/replicate. The results demonstrated that, the average of egg yield per laying duck in experimental group was higher 48.85 eggs/week/240 ducks as compare to control group, the rate of egg-lay in experimental group showed higher 2.90%. The average of egg weight in experimental group was 76.46 g/egg, whereas 74.80 g/egg in control group. The rate of egg shell, egg yolk in experimental group also showed higher than control group, while egg-thick and egg shaped between two groups showed no different significance (p>0.01). Feed conversion ratio/10 eggs in experimental group reduced 0.39kg as compare with control group. The rate of embryo and hatching in experimental group showed higher than in control group. In conclusion, supplementation of Actisaf product showed good effiency in increasing of egg yield, rate of egg shell, rate of egg yolk, embryo and hatching, contributed in improvement of egg production and egg quality in layer ducks.