Genetis polymorphirms of Hsp70 gene in some chicken breeds raised in Viet Nam
The Hsp70 gene encodes a heat shock protein with 70kDa molecule weigh. The Hsp70 protein is a family of chaperone molecules that promote protein foiding and participate in many cellular functions, in particular they protect certain proteins and cells from environmental stressors. This study was conducted to evaluate the genetic polymorphism of Hsp70 gene in four chicken breeds (Ri, TP, HA and LV chicken). Two polymorphic sites in the beginning of coding region of Hsp70 gene, one transition from A (Adenine) to G (Guanine) on position 258 and one transversion from C (Cytosine) to G (Guanine) on position 276, were analyzed by sequencing method. The 648 base pairs fragment of the Hsp70 gene was amplified from four chicken breeds (500 samples/breed) and sequenced directly. The results identified 4 haplotypes (H1, H2, H3 and H4) and 9 genotypes in total samples. Genetic diversity at position A258G and C276G showed the highest in Ri chicken with 9 genotypes: H1H1(2,9%), H1H2 (8,2%), H1H3 (7,5%), H1H4/H2H3 (34,4%), H2H2 (6,8%), H2H4 (3,2%), H3H3 (27,2%), H3H4 (7,5%), H4H4 (2,2%); followed by TP chicken with 9 genotypes: H1H1(2,1%), H1H2 (5,3%), H1H3 (7,4%), H1H4/H2H3 (57,9%), H2H2 (2,1%), H2H4 (1,1%), H3H3 (24,2%); LV chicken with 9 genotype: H1H1(8,4%), H1H2 (3,7%), H1H3 (11,6%), H1H4/H2H3 (31,6%), H2H2 (5,3%), H3H3 (27,2%), H3H4 (11,6%); and the lowest genetic diversity in HA breeds with four genotypes: H1H4/H2H3 (64,2%), H2H2 (9,0%), H3H3 (26,8%).
