This study was conducted to investigate the prevalence of bovine abomasal trichinosis in Laisind, Brahman, and Red Sindhi cows. Treatment experiments were conducted with four treatments, each treatment consists of 12 cows (6 males, 6 females) to evaluate the effectiveness of treating bovine abomasal trichinosis using fenbendazole and Portulaca oleracea (Rau Sam, RS). Treatment 1 (fenbendazole), Treatment 2 (RS100) 100g RS/100kg body weight, Treatment 3 (RS200) 200g RS/100kg body weight, Treatment 4 (RS300) 300g RS/100kg body weight. Results showed that the prevalence of bovine abomasal trichinosis in Laisind cows (43.06%) was higher than Brahman cows (32.05%) and Red Sindhi cows (21.05%) (p<0.05). Treatment 4 (RS300) showed 100% effective bovine abomasal trichinosis, were safe for cows’ health, and resulted in better weight gain compared to Treatment 1 (fenbendazole), Treatment 2 (RS100), and Treatment 3 (RS200).