Investigation of the inhibition of brown dog ticks Rhipicephalus sanguineus by the essential oil of Zingiber officinale and the essential oil of Citrus nobilis peel in vitro
This study was conducted to evaluate the efficacy of self-extracted Citrus nobilis peel essential oil and Zingiber officinale essential oil in inhibiting the dog tick, Rhipicephalus sanguineus. The experiment was designed with two treatments: Treatment 1 utilized 1 ml of self-extracted Citrus nobilis peel essential oil applied to five ticks in porcelain dishes, with three replicates; Treatment 2 followed the same procedure using 1 ml of self-extracted Zingiber officinale essential oil. The mortality rate in both treatments was monitored and recorded at one-minute intervals. The results indicated that Citrus nobilis peel essential oil achieved a 40% inhibition rate at the 7th minute, whereas Zingiber officinale essential oil reached the same rate at the 9th minute. Furthermore, 100% mortality was recorded at the 9th minute for Citrus nobilis peel essential oil treatment, compared to the 13th minute for Zingiber officinale essential oil. These findings demonstrate that both essential oils are effective against R. sanguineus, with the Citrus nobilis peel extract exhibiting a faster acaricidal action. However, the efficacy of both oils increased progressively with exposure time. In conclusion, both self-extracted orange peel and ginger essential oils serve as viable alternatives for controlling R. sanguineus, contributing to the reduction of chemical acaricide reliance.
