The study aimes to identify some common virulence genes of Diarrhoeagenic Escherichia coli (DEC) in domestic chicken flocks, which are important causative agents of diarrhea in the world. We have collected 60 samples feces of chickens with diarrhea from Thai Nguyen and Nam Dinh provinces. The rate of isolation of E. coli was 100%. By PCR method, we have determined that all of the isolated strains carried the 16S rRNA genes; only 6,67% of the strains carried the stx2 gene and none of the strains carried the escV, ent, stx1 and eaeA genes. The results showed that feces of chicken is a potential source of DEC, which can contaminate chicken carcasses during slaughter and pose a health risk for consumers.