Researched on biological characteristics, collected worker bee samples to classify the meliponini for conservation, researched on exploitation, developed genetic resources of meliponini and prevented the risk of degradation and lead to loss gen source. The surveilance was in 6 provinces in the Northern midland and mountainous region (Hoa Binh, Dien Bien, Son La, Lai Chau, Bac Kan, Bac Giang), interviewed 540 people, 535 people of which have kept and keeping meliponini. Meliponini were distributed widely in terms of habitats and altitudes, such as in old forests, in tree hollows, housewall hollows, walls, rock hollows and some lived in termite nests. In some areas in Lai Chau, Dien Bien they lived in the columns of houses, or on the ground of households, distributed from an altitude of 29m to 1736m above sea level. Honey of meliponini was not high, from 306g to 463g/herd/year. With a total of 566 nests that have been caught from the wild, the current number of nests is 129, and 22 of which have been raised for 2 years or more, account for 3.88%, the remaining 544 colonies were raised for less than one year. There were herds when brought back to raise for 2 months, they were leaved away. 3 breeds have been identified (Lepidotrigona, Lisotrigona, Tetragonula) with 08 subspecies (Lepidotrigona flavibasis, Lepidotrigona sp1, Lisotrigona carpenter, Tetragonula sp 2, Tetragonula collina, Tetragonula sp 3, Tetragonula sp 4, Tetragonula sp 5), especially discovered 01 breed was consider to be a new breed published in the prestigious international journal Zookey 1089; 53-72 (2022).