The objective of this research to evaluation of Spirulina platensis supplementation on lactating dairy cow performance, feed intake, milk production and lameness in dairy cattle. The trial was conducted on 15 first lactation dairy cows in Bavi cattle and Forage Research Center from on 60 day of period. All animal was randomized in 3 groups of the experimental with supplementation 0, 40 and 200 g per cow per day of Spirulina. The animals was analysed of body weight, everyday milk production. The sample of milk was analysis of chemical composition, fatty acids (SFA and USFA). The results were showed that: Spirulina platensis supplementation of 40 and 200 g/cow/day had no affected on dry master intake, milk production, milk fat, milk protein and milk solids, while slily encreasing the body condition score of cattle. Inadtion, the supplementation of 40 g Spirulina platensis was significant decreased the somatic cell count in milk and changing the unsaturated fatty acid, and fatty acid potentiol for human health. Base on this study it coutd be suggedted thats supplementation of 40 g Spirulina platensis may effect on structure of milk fatty acid towards healthy for consumer.