Application of total Aflatoxin content (B1, B2, G1, G2) determination method by immunoaffinity columnon high-performance liquid chromatography (HPLC)
The results from applying the method of determining the total Aflatoxin content (B1, B2, G1, G2) in animal feeds using High-Performance Liquid Chromatography (HPLC) have built a linear calibration curve between concentration of Aflatoxin standards (B1, B2, G1, G2) and the peak area of chromatograms with a high linear coefficient R2 from 0.99953 to 1.00. Since then, the use of this calibration curve to calculate and determine the total Aflatoxin content (B1, B2, G1, G2) in 3 sample groups: corn grain, feed concentrate and feed mixture for pigs. These samples were representative of the animal feed production facilities in Hung Yen. The total Aflatoxin content results (B1, B2, G1, G2) in these samples were very low and were used as base samples to determine the recovery by adding the Aflatoxin standard stock 2600 ppb. The recovery of total Aflatoxin standard on sample bases was high, which was performed by two technicians in the same time period, on the same analyzer and on the same sample group. The results of the corresponding recovery were 94.60% for corn grain base samples, 83.76% for feed mixture base samples for pigs and 87.43% for feed concentrate. These results confirmed that the total Aflatoxin content analysis procedure (B1, B2, G1, G2) in animal feeds using the immunoaffinity column on High-Performance Liquid Chromatography (HPLC) was accurate and consistent with analytical equipment and skills in Vietnam.
